Figure 1. Western blot analysis of GRB10 using anti-GRB10 antibody (M01663). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hek293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat NRK whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GRB10 antigen affinity purified monoclonal antibody (Catalog # M01663) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for GRB10 at approximately 67KD. The expected band size for GRB10 is at 67KD.
Figure 1. Western blot analysis of GRB10 using anti-GRB10 antibody (M01663). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hek293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat NRK whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GRB10 antigen affinity purified monoclonal antibody (Catalog # M01663) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for GRB10 at approximately 67KD. The expected band size for GRB10 is at 67KD.
Anti-GRB10 Antibody Picoband(r) (monoclonal, 5H7)
M01663-DYLIGHT488
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityHuman, Monkey, Rat
TargetGRB10
Overview
- SupplierBoster Bio
- Product NameAnti-GRB10 Antibody Picoband(r) (monoclonal, 5H7)
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, Western Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID5H7
- Concentration500 ug/ml
- ConjugateDyLight 488
- Gene ID2887
- Target nameGRB10
- Target descriptiongrowth factor receptor bound protein 10
- Target synonymsGrb-10; GRB10 adapter protein; GRB10 adaptor protein; GRB-IR; growth factor receptor-bound protein 10; insulin receptor-binding protein Grb-IR; IRBP; maternally expressed gene 1; MEG1; RSS
- HostMouse
- IsotypeIgG1
- Protein IDQ13322
- Protein NameGrowth factor receptor-bound protein 10
- Scientific DescriptionBoster Bio Anti-GRB10 Antibody Picoband® (monoclonal, 5H7) catalog # M01663. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Monkey, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Monkey, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203