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Western Blot analysis of 3T3 cells using GRB10 Polyclonal Antibody
Western Blot analysis of 3T3 cells using GRB10 Polyclonal Antibody
Western Blot analysis of 3T3 cells using GRB10 Polyclonal Antibody

GRB10 Antibody

CSB-PA002806
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGRB10
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Overview

  • Supplier
    Cusabio
  • Product Name
    GRB10 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2887
  • Target name
    GRB10
  • Target description
    growth factor receptor bound protein 10
  • Target synonyms
    GRB-IR, Grb-10, IRBP, MEG1, RSS, growth factor receptor-bound protein 10, GRB10 adapter protein, GRB10 adaptor protein, insulin receptor-binding protein Grb-IR, maternally expressed gene 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13322
  • Protein Name
    Growth factor receptor-bound protein 10
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GRB10 using anti-GRB10 antibody (A01663-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human U87 whole cell lysates, Lane 7: human HEL whole cell lysates, Lane 8: rat liver tissue lysates, Lane 9: rat brain tissue lysates, Lane 10: mouse liver tissue lysates, Lane 11: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRB10 antigen affinity purified polyclonal antibody (Catalog # A01663-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRB10 at approximately 76 kDa. The expected band size for GRB10 is at 67 kDa.
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