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Immunohistochemical staining of human tonsil shows strong nuclear immunoreactivity in lymphoid cells.
Immunohistochemical staining of human tonsil shows strong nuclear immunoreactivity in lymphoid cells.
Immunohistochemical staining of human tonsil shows strong nuclear immunoreactivity in lymphoid cells.

Anti-H2AFY Antibody

AMAB91347
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMACROH2A1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-H2AFY Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL5245
  • Conjugate
    Unconjugated
  • Gene ID9555
  • Target name
    MACROH2A1
  • Target description
    macroH2A.1 histone
  • Target synonyms
    H2A.y, H2A/y, H2AF12M, H2AFY, MACROH2A1.1, mH2A1, macroH2A1.2, core histone macro-H2A.1, H2A histone family member Y, histone H2A.y, histone macroH2A1, histone macroH2A1.1, histone macroH2A1.2, medulloblastoma antigen MU-MB-50.205
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO75367
  • Protein Name
    Core histone macro-H2A.1
  • Scientific Description
    Synthetic Peptide
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of H2AFY/MACROH2A1 using anti-H2AFY/MACROH2A1 antibody (A04635-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-H2AFY/MACROH2A1 antigen affinity purified polyclonal antibody (Catalog # A04635-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for H2AFY/MACROH2A1 at approximately 39 kDa. The expected band size for H2AFY/MACROH2A1 is at 40 kDa.
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