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Western blot All lanes: H2AFY antibody at 1.43microg/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa
Western blot All lanes: H2AFY antibody at 1.43microg/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa
Western blot All lanes: H2AFY antibody at 1.43microg/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa

H2AFY Antibody

CSB-PA010098DSR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMACROH2A1
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Overview

  • Supplier
    Cusabio
  • Product Name
    H2AFY Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9555
  • Target name
    MACROH2A1
  • Target description
    macroH2A.1 histone
  • Target synonyms
    core histone macro-H2A.1; H2A histone family member Y; H2A.y; H2A/y; H2AF12M; H2AFY; histone H2A.y; histone macroH2A1; histone macroH2A1.1; histone macroH2A1.2; MACROH2A1.1; macroH2A1.2; medulloblastoma antigen MU-MB-50.205; mH2A1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75367
  • Protein Name
    Core histone macro-H2A.1
  • Scientific Description
    Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of H2AFY/MACROH2A1 using anti-H2AFY/MACROH2A1 antibody (A04635-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-H2AFY/MACROH2A1 antigen affinity purified polyclonal antibody (Catalog # A04635-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for H2AFY/MACROH2A1 at approximately 39 kDa. The expected band size for H2AFY/MACROH2A1 is at 40 kDa.
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