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Immunohistochemical staining of human Cerebral cortex shows moderate nuclear positivity in glial and neuronal cells.
Immunohistochemical staining of human Cerebral cortex shows moderate nuclear positivity in glial and neuronal cells.
Immunohistochemical staining of human Cerebral cortex shows moderate nuclear positivity in glial and neuronal cells.

Anti-HDAC9 Antibody

HPA028926
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetHDAC9
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HDAC9
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9734
  • Target name
    HDAC9
  • Target description
    histone deacetylase 9
  • Target synonyms
    HD7; HD7b; HD9; HDAC; HDAC7; HDAC7B; HDAC9B; HDAC9FL; HDRP; histone deacetylase 4/5-related protein; histone deacetylase 7B; histone deacetylase 9; MEF-2 interacting transcription repressor (MITR) protein; MITR
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UKV0
  • Protein Name
    Histone deacetylase 9
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HDAC9 using anti-HDAC9 antibody (A02177-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse RAW264.7 whole cell lysates, Lane 6: human THP-1 whole cell lysates, Lane 7: human HL-60 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC9 antigen affinity purified polyclonal antibody (Catalog # A02177-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC9 at approximately 150KD. The expected band size for HDAC9 is at 150KD.
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Lane 1: K562 Cells; Lane 2: HepG2 Cells; Lane 3: Raji Cells; Probed with HDAC9 (2C9) Monoclonal Antibody (bsm-54186R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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