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Western Blot analysis of HepG2 cells using HDAC5/9 Polyclonal Antibody
Western Blot analysis of HepG2 cells using HDAC5/9 Polyclonal Antibody
Western Blot analysis of HepG2 cells using HDAC5/9 Polyclonal Antibody

HDAC5/HDAC9 Antibody

CSB-PA002890
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetHDAC9
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Overview

  • Supplier
    Cusabio
  • Product Name
    HDAC5/HDAC9 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9734
  • Target name
    HDAC9
  • Target description
    histone deacetylase 9
  • Target synonyms
    ARCND4, HD7, HD7b, HD9, HDAC, HDAC7, HDAC7B, HDAC9B, HDAC9FL, HDRP, MITR, histone deacetylase 9, MEF-2 interacting transcription repressor (MITR) protein, histone deacetylase 4/5-related protein, histone deacetylase 7B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UKV0
  • Protein Name
    Histone deacetylase 9
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HDAC9 using anti-HDAC9 antibody (A02177-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse RAW264.7 whole cell lysates, Lane 6: human THP-1 whole cell lysates, Lane 7: human HL-60 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC9 antigen affinity purified polyclonal antibody (Catalog # A02177-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC9 at approximately 150KD. The expected band size for HDAC9 is at 150KD.
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Lane 1: K562 Cells; Lane 2: HepG2 Cells; Lane 3: Raji Cells; Probed with HDAC9 (2C9) Monoclonal Antibody (bsm-54186R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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