Immunohistochemistry staining of 293T cells expressing His-Tag nuclear protein X, using anti-His-Tag antibody, RM146.
anti-His-Tag, Rabbit Monoclonal (RM146)
REV-31-1048-00
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityAll Species
Overview
- SupplierRevMAb Biosciences
- Product Nameanti-His-Tag, Rabbit Monoclonal (RM146)
- Delivery Days Customer5
- Antibody SpecificityThis antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
- ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDRM146
- Concentration1 mg/ml
- FormulationLiquid
- HostRabbit
- IsotypeIgG
- Scientific DescriptionHis-tag is a series of six to nine histidine residues fused to either the carboxyl or amino terminus of a recombinant protein. The small size of the His-tag, compared with other common epitope tags, makes it less likely to obstruct the target proteins structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions allows for the simple purification and detection of His-tagged proteins. His-tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe. This His-tag antibody is also available biotin-labeled to meet all your research needs. - Recombinant Antibody. This antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells. Applications: WB, IP, ICC, FACS, IHC. Source: Rabbit. Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. His-tag is a series of six to nine histidine residues fused to either the carboxyl or amino terminus of a recombinant protein. The small size of the His-tag, compared with other common epitope tags, makes it less likely to obstruct the target proteins structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions allows for the simple purification and detection of His-tagged proteins. His-tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe. This His-tag antibody is also available biotin-labeled to meet all your research needs.
- ReactivityAll Species
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203