Immunostaining of FFPE 293 cells overexpressing a cytoplasmic or nuclear protein, using anti-His-tag rabbit monoclonal antibody (clone RM146X) at 4ng/ml.
Immunostaining of FFPE 293 cells overexpressing a cytoplasmic or nuclear protein, using anti-His-tag rabbit monoclonal antibody (clone RM146X) at 4ng/ml.
anti-His-Tag, Rabbit Monoclonal (RM146X)
REV-38-1048-00
Product group Antibodies
Overview
- SupplierRevMAb Biosciences
- Product Nameanti-His-Tag, Rabbit Monoclonal (RM146X)
- Delivery Days Customer10
- CertificationResearch Use Only
- ClonalityMonoclonal
- Scientific DescriptionHis-tag is a series of six to nine histidine residues fused to either the carboxyl or amino terminus of a recombinant protein. The small size of the His-tag, compared with other common epitope tags, makes it less likely to obstruct the target proteins structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions allows for the simple purification and detection of His-tagged proteins. His-tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe. This His-tag antibody is also available biotin-labeled to meet all your research needs. - Recombinant Antibody. RM146X reacts to recombinant proteins containing the His-Tag fused to either the amino or carboxy terminus. Very low non-specific reactivity with endogenous proteins in mammalian or bacteria cells, compared with other anti-HisTag antibodies. Isotype: Rabbit IgG. Immunogen: 6xHis-Tag coupled to KLH. Applications: ELISA, ICC, IHC, IP, WB. Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. His-tag is a series of six to nine histidine residues fused to either the carboxyl or amino terminus of a recombinant protein. The small size of the His-tag, compared with other common epitope tags, makes it less likely to obstruct the target proteins structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions allows for the simple purification and detection of His-tagged proteins. His-tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe. This His-tag antibody is also available biotin-labeled to meet all your research needs.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203