Immunohistochemical analysis of paraffin-embedded rat lung, using Histone H2AX Antibody(M00241-1) H2AFX was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-H2AFX Antibody (M00241-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Histone H2A.X Rabbit Monoclonal Antibody
M00241-1
Product group Antibodies
Overview
- SupplierBoster Bio
- Product NameAnti-Histone H2A.X Rabbit Monoclonal Antibody
- Delivery Days Customer9
- CertificationResearch Use Only
- UNSPSC12352203