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Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.
Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.
Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.

Anti-HLA-DRB1 Antibody

HPA043151
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetHLA-DRB1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HLA-DRB1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3123
  • Target name
    HLA-DRB1
  • Target description
    major histocompatibility complex, class II, DR beta 1
  • Target synonyms
    DRB1; HLA class II histocompatibility antigen, DR-1 beta chain; HLA-DR1B; HLA-DRB; human leucocyte antigen DRB1; lymphocyte antigen DRB1; major histocompatibility complex, class II, DR beta 1 precursor; MHC class II HLA-DR beta 1 chain; SS1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01911
  • Protein Name
    HLA class II histocompatibility antigen, DRB1-15 beta chain
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of HLA Class II DRB1 using anti-HLA Class II DRB1 antibody (A00568). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji whole cell lysate, Lane 2: human Raji whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA Class II DRB1 antigen affinity purified polyclonal antibody (Catalog # A00568) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLA Class II DRB1 at approximately 30KD. The expected band size for HLA Class II DRB1 is at 30KD.
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