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HLA-DRB1 Antibody (YA1487, Rabbit)

HY-P81742
MedChem Express
TargetHLA-DRB1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    HLA-DRB1 Antibody (YA1487, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3123
  • Target name
    HLA-DRB1
  • Target description
    major histocompatibility complex, class II, DR beta 1
  • Target synonyms
    DRB1, HLA-DR1B, HLA-DRB, SS1, major histocompatibility complex, class II, DR beta 1 precursor, HLA class II histocompatibility antigen, DR-1 beta chain, MHC class II HLA-DR beta 1 chain, human leucocyte antigen DRB1, lymphocyte antigen DRB1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    HLA-DRB1 Antibody (YA1487) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1487), targeting HLA DRB1, with a predicted molecular weight of 30 kDa (observed band size: 30 kDa). HLA-DRB1 Antibody (YA1487) can be used for WB, IHC-P, ICC/IF experiment in human background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HLA Class II DRB1 using anti-HLA Class II DRB1 antibody (A00568). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji whole cell lysate, Lane 2: human Raji whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA Class II DRB1 antigen affinity purified polyclonal antibody (Catalog # A00568) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLA Class II DRB1 at approximately 30KD. The expected band size for HLA Class II DRB1 is at 30KD.
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