Figure 1. Western blot analysis of HMG4 using anti-HMG4 antibody (PB9633). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Mouse Liver Tissue Lysate at 50ug, Lane 2: Mouse Kidney Tissue Lysate at 50ug, Lane 3: Mouse Testis Tissue Lysate at 50ug, Lane 4: 22RV1 Whole Cell Lysate at 40ug, Lane 5: MCF-7 Whole Cell Lysate at 40ug, Lane 6: NIH3T3 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMG4 antigen affinity purified polyclonal antibody (Catalog # PB9633) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HMG4 at approximately 23 kDa. The expected band size for HMG4 is at 23 kDa.