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Paraformaldehyde-fixed, paraffin embedded Rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HMG4/HMGB3 Polyclonal Antibody, Unconjugated (bs-8809R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HMG4/HMGB3 Polyclonal Antibody, Unconjugated (bs-8809R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HMG4/HMGB3 Polyclonal Antibody, Unconjugated (bs-8809R) at 1:200 overnight at 4°C, DAB staining.

HMG4/HMGB3 Polyclonal Antibody

BS-8809R
Bioss
ApplicationsImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetHMGB3
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Overview

  • Supplier
    Bioss
  • Product Name
    HMG4/HMGB3 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:100-500), IF(IF=1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3149
  • Target name
    HMGB3
  • Target description
    high mobility group box 3
  • Target synonyms
    HMG-2a, HMG-4, HMG2A, HMG4, high mobility group protein B3, high mobility group protein 2a, high-mobility group (nonhistone chromosomal) protein 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15347
  • Protein Name
    High mobility group protein B3
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of HMG4 using anti-HMG4 antibody (PB9633). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Mouse Liver Tissue Lysate at 50ug, Lane 2: Mouse Kidney Tissue Lysate at 50ug, Lane 3: Mouse Testis Tissue Lysate at 50ug, Lane 4: 22RV1 Whole Cell Lysate at 40ug, Lane 5: MCF-7 Whole Cell Lysate at 40ug, Lane 6: NIH3T3 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMG4 antigen affinity purified polyclonal antibody (Catalog # PB9633) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HMG4 at approximately 23 kDa. The expected band size for HMG4 is at 23 kDa.
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