Western blot: 20 microg of COLO205, HCT116, HT29, SW480 and SW620 cell lysates stained with ARG10756 anti-HMGB1 antibody [1F3] (1:2000) and ARG65680 anti-GAPDH antibody (1:10000).
![Immunocytochemistry: HeLa cells grown in tissue culture and stained with ARG10756 anti-HMGB1 antibody [1F3] (red) and co-stained with chicken polyclonal antibody to Vimentin (green); DNA (blue). ARG10756 anti-HMGB1 antibody [1F3] reveals strong nuclear staining which overlaps with the DNA stain.](https://www.arigobio.com/files/product/12556/ARG10756_ICC_1.jpg "Immunocytochemistry: HeLa cells grown in tissue culture and stained with ARG10756 anti-HMGB1 antibody [1F3] (red) and co-stained with chicken polyclonal antibody to Vimentin (green); DNA (blue). ARG10756 anti-HMGB1 antibody [1F3] reveals strong nuclear staining which overlaps with the DNA stain.")
![Immunofluorescence: HeLa cells stained with ARG10756 anti-HMGB1 antibody [1F3] (red) at 1:2000 dilution and costained with Rabbit pAb to GAPDH (green) at 1:2000 dilution.](https://www.arigobio.com/files/product/12556/ARG10756_IF_190729.jpg "Immunofluorescence: HeLa cells stained with ARG10756 anti-HMGB1 antibody [1F3] (red) at 1:2000 dilution and costained with Rabbit pAb to GAPDH (green) at 1:2000 dilution.")
![Western blot: NIH/3T3, C6, HEK293, HeLa and SH-SY5Y cell lysates stained with ARG10756 anti-HMGB1 antibody [1F3] (green) at 1:2000 dilution.](https://www.arigobio.com/files/product/12556/ARG10756_WB_190729.jpg "Western blot: NIH/3T3, C6, HEK293, HeLa and SH-SY5Y cell lysates stained with ARG10756 anti-HMGB1 antibody [1F3] (green) at 1:2000 dilution.")
![Western blot: 30 microg of 293T and 3T3 cell lysates stained with ARG10756 anti-HMGB1 antibody [1F3] at 1:1000 dilution.](https://www.arigobio.com/files/product/12556/ARG10756_WB_Sheet_Rev_293T_3T3_170511.jpg "Western blot: 30 microg of 293T and 3T3 cell lysates stained with ARG10756 anti-HMGB1 antibody [1F3] at 1:1000 dilution.")
Western blot: 20 microg of COLO205, HCT116, HT29, SW480 and SW620 cell lysates stained with ARG10756 anti-HMGB1 antibody [1F3] (1:2000) and ARG65680 anti-GAPDH antibody (1:10000).
anti-HMGB1 antibody [1F3]
ARG10756
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityHuman
TargetHMGB1
Overview
- SupplierArigo Biolaboratories
- Product Nameanti-HMGB1 antibody [1F3]
- Delivery Days Customer23
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID1F3
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3146
- Target nameHMGB1
- Target descriptionhigh mobility group box 1
- Target synonymsAmphoterin; high mobility group protein B1; high-mobility group (nonhistone chromosomal) protein 1; HMG1; HMG-1; HMG3; SBP-1; Sulfoglucuronyl carbohydrate binding protein
- HostMouse
- IsotypeIgG2b
- Scientific DescriptionHMGB1 is a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203