ELISA: ARG65863 anti-HMGB1 antibody [SQab1711] at 1:5000 were used for detecting HMGB1. 5 microg/ml of HMGB1, HMGB2 and BSA proteins were coated onto ELISA plate.
![Flow Cytometry: HuH-7 cell and HuH-7 shHMGB1 cell stained with ARG65863 anti-HMGB1 antibody [SQab1711] at 1 microg/ml dilution.](https://www.arigobio.com/files/product/13011/ARG65863_FACS_HuH7.jpg "Flow Cytometry: HuH-7 cell and HuH-7 shHMGB1 cell stained with ARG65863 anti-HMGB1 antibody [SQab1711] at 1 microg/ml dilution.")
![Immunofluorescence: 293T cells stained with ARG65863 anti-HMGB1 antibody [SQab1711] at 5 microg/ml dilution.](https://www.arigobio.com/files/product/13011/ARG65863_IF_293T_v2.jpg "Immunofluorescence: 293T cells stained with ARG65863 anti-HMGB1 antibody [SQab1711] at 5 microg/ml dilution.")
![Immunohistochemistry: Paraffin-embedded Human cervical cancer stained with ARG65863 anti-HMGB1 antibody [SQab1711] at 10 microg/ml dilution.](https://www.arigobio.com/files/product/13011/ARG65863_IHC_HuCervicalCancer_v2.jpg "Immunohistochemistry: Paraffin-embedded Human cervical cancer stained with ARG65863 anti-HMGB1 antibody [SQab1711] at 10 microg/ml dilution.")
![Immunohistochemistry: Paraffin-embedded Mouse kidney stained with ARG65863 anti-HMGB1 antibody [SQab1711] at 10 microg/ml dilution.](https://www.arigobio.com/files/product/13011/ARG65863_IHC_MsKidney_v2.jpg "Immunohistochemistry: Paraffin-embedded Mouse kidney stained with ARG65863 anti-HMGB1 antibody [SQab1711] at 10 microg/ml dilution.")
![Immunohistochemistry: Paraffin-embedded Mouse liver stained with ARG65863 anti-HMGB1 antibody [SQab1711] at 10 microg/ml dilution.](https://www.arigobio.com/files/product/13011/ARG65863_IHC_MsLiver_v2.jpg "Immunohistochemistry: Paraffin-embedded Mouse liver stained with ARG65863 anti-HMGB1 antibody [SQab1711] at 10 microg/ml dilution.")
![Immunohistochemistry: Paraffin-embedded Rat liver stained with ARG65863 anti-HMGB1 antibody [SQab1711] at 10 microg/ml dilution.](https://www.arigobio.com/files/product/13011/ARG65863_Rat_Liver_IHC-P_v211.jpg "Immunohistochemistry: Paraffin-embedded Rat liver stained with ARG65863 anti-HMGB1 antibody [SQab1711] at 10 microg/ml dilution.")
![Western blot: 1) 50 ng of HMGB1 (Purified from E. coli) and 2) 100 ng of HMGB2 (Purified from E.coli) stained with ARG65863 anti-HMGB1 antibody [SQab1711] at 1:5000 dilution.](https://www.arigobio.com/files/product/13011/ARG65863_WB_2Protein_170210.jpg "Western blot: 1) 50 ng of HMGB1 (Purified from E. coli) and 2) 100 ng of HMGB2 (Purified from E.coli) stained with ARG65863 anti-HMGB1 antibody [SQab1711] at 1:5000 dilution.")
![Western blot: 50 microg of 1) HuH-7 and 2) HuH-7 shHMGB1 cell lysates stained with ARG65863 anti-HMGB1 antibody [SQab1711] at 1:5000 dilution.](https://www.arigobio.com/files/product/13011/ARG65863_WB_Huh7_170210.jpg "Western blot: 50 microg of 1) HuH-7 and 2) HuH-7 shHMGB1 cell lysates stained with ARG65863 anti-HMGB1 antibody [SQab1711] at 1:5000 dilution.")
![Western blot: 20 microg of 1) Mouse spleen and 2) Rat spleen lysates stained with ARG65863 anti-HMGB1 antibody [SQab1711] at 1:5000 dilution.](https://www.arigobio.com/files/product/13011/ARG65863_WB_MRspleen_170210.jpg "Western blot: 20 microg of 1) Mouse spleen and 2) Rat spleen lysates stained with ARG65863 anti-HMGB1 antibody [SQab1711] at 1:5000 dilution.")
ELISA: ARG65863 anti-HMGB1 antibody [SQab1711] at 1:5000 were used for detecting HMGB1. 5 microg/ml of HMGB1, HMGB2 and BSA proteins were coated onto ELISA plate.
anti-HMGB1 antibody [SQab1711]
ARG65863
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetHMGB1
Overview
- SupplierArigo Biolaboratories
- Product Nameanti-HMGB1 antibody [SQab1711]
- Delivery Days Customer23
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDSQab1711
- ConjugateUnconjugated
- Gene ID3146
- Target nameHMGB1
- Target descriptionhigh mobility group box 1
- Target synonymsAmphoterin; high mobility group protein B1; high-mobility group (nonhistone chromosomal) protein 1; HMG1; HMG-1; HMG3; SBP-1; Sulfoglucuronyl carbohydrate binding protein
- HostMouse
- IsotypeIgG2b
- Scientific DescriptionHMGB1 is a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
- Storage Instruction-20°C
- UNSPSC12352203