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Anti-HMGCS1 Antibody

144-03916
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetHMGCS1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-HMGCS1 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3157
  • Target name
    HMGCS1
  • Target description
    3-hydroxy-3-methylglutaryl-CoA synthase 1
  • Target synonyms
    3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase; 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble); 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble); HMGCS; hydroxymethylglutaryl-CoA synthase, cytoplasmic
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01581
  • Protein Name
    Hydroxymethylglutaryl-CoA synthase, cytoplasmic
  • Scientific Description
    HMGCS1 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HMGCS1 using anti-HMGCS1 antibody (A05313-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMGCS1 antigen affinity purified polyclonal antibody (Catalog # A05313-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HMGCS1 at approximately 57 kDa. The expected band size for HMGCS1 is at 57 kDa.
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