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Anti-HNRNPA1 Antibody

144-07491
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetHNRNPA1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-HNRNPA1 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3178
  • Target name
    HNRNPA1
  • Target description
    heterogeneous nuclear ribonucleoprotein A1
  • Target synonyms
    ALS19; ALS20; epididymis secretory sperm binding protein; helix-destabilizing protein; heterogeneous nuclear ribonucleoprotein A1; heterogeneous nuclear ribonucleoprotein A1B protein; heterogeneous nuclear ribonucleoprotein B2 protein; heterogeneous nuclear ribonucleoprotein core protein A1; hnRNP A1; hnRNP core protein A1-like 3; hnRNP-A1; HNRPA1; HNRPA1L3; IBMPFD3; nuclear ribonucleoprotein particle A1 protein; putative heterogeneous nuclear ribonucleoprotein A1-like 3; single-strand DNA-binding protein UP1; single-strand RNA-binding protein; UP 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09651
  • Protein Name
    Heterogeneous nuclear ribonucleoprotein A1
  • Scientific Description
    HNRNPA1 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HnRNP A1 using anti-HnRNP A1 antibody (A01476). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HnRNP A1 antigen affinity purified polyclonal antibody (Catalog # A01476) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HnRNP A1 at approximately 36 kDa. The expected band size for HnRNP A1 is at 39 kDa.
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