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Western Blot analysis of COLO205 cells using hnRNP A1 Polyclonal Antibody
Western Blot analysis of COLO205 cells using hnRNP A1 Polyclonal Antibody
Western Blot analysis of COLO205 cells using hnRNP A1 Polyclonal Antibody

HNRNPA1 Antibody

CSB-PA002942
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHNRNPA1
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Overview

  • Supplier
    Cusabio
  • Product Name
    HNRNPA1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3178
  • Target name
    HNRNPA1
  • Target description
    heterogeneous nuclear ribonucleoprotein A1
  • Target synonyms
    ALS19, ALS20, HNRPA1, HNRPA1L3, IBMPFD3, MPD3, UP 1, hnRNP A1, hnRNP-A1, heterogeneous nuclear ribonucleoprotein A1, epididymis secretory sperm binding protein, helix-destabilizing protein, heterogeneous nuclear ribonucleoprotein A1B protein, heterogeneous nuclear ribonucleoprotein B2 protein, heterogeneous nuclear ribonucleoprotein core protein A1, hnRNP core protein A1-like 3, nuclear ribonucleoprotein particle A1 protein, putative heterogeneous nuclear ribonucleoprotein A1-like 3, single-strand DNA-binding protein UP1, single-strand RNA-binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09651
  • Protein Name
    Heterogeneous nuclear ribonucleoprotein A1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of HnRNP A1 using anti-HnRNP A1 antibody (A01476). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HnRNP A1 antigen affinity purified polyclonal antibody (Catalog # A01476) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HnRNP A1 at approximately 36 kDa. The expected band size for HnRNP A1 is at 39 kDa.
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