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Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm.
Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm.
Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm.

Anti-HOXA11 Antibody

HPA006770
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetHOXA11
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HOXA11
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3207
  • Target name
    HOXA11
  • Target description
    homeobox A11
  • Target synonyms
    homeo box 1I; homeobox protein Hox-1I; homeobox protein HOXA11; homeobox protein Hox-A11; HOX1; HOX1I; RUSAT1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31270
  • Protein Name
    Homeobox protein Hox-A11
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HOXA11 using anti-HOXA11 antibody (PB9218). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. lane 1: Recombinant Human HOXA11 Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXA11 antigen affinity purified polyclonal antibody (Catalog # PB9218) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXA11 at approximately 38KD. The expected band size for HOXA11 is at 38KD.
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