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HOXA11 Antibody

LS-C332310
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetHOXA11
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    HOXA11 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 34kDa, while the observed MW by Western blot was 35kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:200 - 1:500) The predicted MW is 34kDa, while the observed MW by Western blot was 35kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3207
  • Target name
    HOXA11
  • Target description
    homeobox A11
  • Target synonyms
    homeo box 1I; homeobox protein Hox-1I; homeobox protein HOXA11; homeobox protein Hox-A11; HOX1; HOX1I; RUSAT1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HOXA11 using anti-HOXA11 antibody (PB9218). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. lane 1: Recombinant Human HOXA11 Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXA11 antigen affinity purified polyclonal antibody (Catalog # PB9218) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXA11 at approximately 38KD. The expected band size for HOXA11 is at 38KD.
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