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Western Blot analysis of 293T-UV cells using HoxA11/D11 Polyclonal Antibody
Western Blot analysis of 293T-UV cells using HoxA11/D11 Polyclonal Antibody
Western Blot analysis of 293T-UV cells using HoxA11/D11 Polyclonal Antibody

HOXA11/HOXD11 Antibody

CSB-PA002955
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetHOXA11
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Overview

  • Supplier
    Cusabio
  • Product Name
    HOXA11/HOXD11 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3207
  • Target name
    HOXA11
  • Target description
    homeobox A11
  • Target synonyms
    HOX1, HOX1I, RUSAT1, homeobox protein Hox-A11, homeo box 1I, homeobox protein HOXA11, homeobox protein Hox-1I
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31270
  • Protein Name
    Homeobox protein Hox-A11
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HOXA11 using anti-HOXA11 antibody (PB9218). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. lane 1: Recombinant Human HOXA11 Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXA11 antigen affinity purified polyclonal antibody (Catalog # PB9218) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXA11 at approximately 38KD. The expected band size for HOXA11 is at 38KD.
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