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Anti-HP1-gamma Antibody

Anti-HP1-gamma Antibody

A40148
Antibodies.com
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-HP1-gamma Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Mouse monoclonal antibody to HP1-gamma
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HP1 gamma using anti-HP1 gamma antibody (A01142). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat spleen tissue lysate, Lane 3: rat testis tissue lysate, Lane 4: rat PC-12 whole cell lysate, Lane 5: mouse brain tissue lysate, Lane 6: mouse brain tissue lysate, Lane 7: mouse testis tissue lysate, Lane 8: mouse HEPA1-6 whole cell lysate, Lane 9: mouse NIH/3T3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HP1 gamma antigen affinity purified polyclonal antibody (Catalog # A01142) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HP1 gamma at approximately 21KD. The expected band size for HP1 gamma is at 21KD.
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