Anti-HPV16 E7 protein [69E2], Human IgG1, kappa

Absolute Antibody

Anti-HPV16 E7 protein [69E2], Human IgG1, kappa

9

The binding characterization of this antibody for HPV E7 protein was done using ELISA. The original IgG2a version of this antibody binds HPV E7 protein with a binding affinity of Kd= 5.6 nM. The anti-HPVE7 protein antibody 79A11 can be used as capture antibody in combination with HRP conjugated 69E2 antibody as a detection antibody in a sandwich ELISA. This antibody can be used for the detection of HPV E7 protein from cells lysates of cervical cancer cell lines CaSki cells and HeLa cells in a western blot. This antibody was also used in the immunocytochemical analysis of CaSki cells and HeLa cells (HPV positive). This antibody was used for the immunohistochemical analysis of HPV16-positive cervical cancer squamous cell carcinoma and HPV18-positive cervical cancer adenocarcinoma. This antibody was also used for the indirect immunofluorescence staining of CaSki cells and HeLa cells. A chemiluminescent immunoassay was prepared using 79A11 as capture and Biotin-69E2 and HRP-Streptavidin for detection (CN111410689).

ImmunoFluorescence, Western Blot, CLIA ChemiLuminecent ImmunoAssay, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry

Research Use Only

Monoclonal

69E2

E7

transforming protein E7

HpV16gp2; transforming protein E7

Human

IgG1

Protein E7

-20°C,2°C to 8°C

12352203