Detection of Human Papillomavirus type 16 E7 protein by lateral flow assay using the monoclonal antibody pair. Capture: Human Papillomavirus type 16 E7 antibody (GTX637228 [HL1647]) Detection: Human Papillomavirus type 16 E7 antibody (GTX637546 [HL1821]) Samples (10 ng) : 1. HPV16 E7 protein (GTX133411-pro) 2. HPV18 E7 protein (GTX133412-pro) 3. Lysis buffer
![Sandwich ELISA detection of recombinant Human Papillomavirus type 16 E7 protein, His tag (GTX133411-pro) using antibodies as below. Capture: Human Papillomavirus type 16 E7 antibody [HL1647] (GTX637228) (5 μg/mL) Detection: HRP-conjugated Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) (1 μg/mL) Please notice that GTX637546 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637228. Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637546/GTX637546_T-44914_20230224_ELISA_PAIR_23030219_967.webp "Sandwich ELISA detection of recombinant Human Papillomavirus type 16 E7 protein, His tag (GTX133411-pro) using antibodies as below. Capture: Human Papillomavirus type 16 E7 antibody [HL1647] (GTX637228) (5 μg/mL) Detection: HRP-conjugated Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) (1 μg/mL) Please notice that GTX637546 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637228. Please contact us for custom HRP-conjugated antibody.")
![Sandwich ELISA detection of recombinant Human Papillomavirus type 16 E7 protein, His tag (GTX133411-pro) using antibodies as below. Capture: Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) (5 μg/mL) Detection: HRP-conjugated Human Papillomavirus type 16 E7 antibody [HL1647] (GTX637228) (1 μg/mL) Please notice that GTX637228 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637546. Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637546/GTX637546_T-44816_20230224_ELISA_PAIR_2_23030219_765.webp "Sandwich ELISA detection of recombinant Human Papillomavirus type 16 E7 protein, His tag (GTX133411-pro) using antibodies as below. Capture: Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) (5 μg/mL) Detection: HRP-conjugated Human Papillomavirus type 16 E7 antibody [HL1647] (GTX637228) (1 μg/mL) Please notice that GTX637228 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637546. Please contact us for custom HRP-conjugated antibody.")
![Human papillomavirus protein (100 ng) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637546/GTX637546_44984_20230512_WB_HPV_Ag_23051702_117.webp "Human papillomavirus protein (100 ng) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637546/GTX637546_44984_20230602_WB_multiplevirus_B_23060622_316.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papillomavirus type 16 E7 antibody [HL1821] (GTX637546) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papilloma virus type 16 E7 antibody [HL1821] (GTX637546) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637546/GTX637546_45474_20240719_WB_25070323_635.webp "Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papilloma virus type 16 E7 antibody [HL1821] (GTX637546) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
Detection of Human Papillomavirus type 16 E7 protein by lateral flow assay using the monoclonal antibody pair. Capture: Human Papillomavirus type 16 E7 antibody (GTX637228 [HL1647]) Detection: Human Papillomavirus type 16 E7 antibody (GTX637546 [HL1821]) Samples (10 ng) : 1. HPV16 E7 protein (GTX133411-pro) 2. HPV18 E7 protein (GTX133412-pro) 3. Lysis buffer
Human Papillomavirus type 16 E7 antibody [HL1821]
GTX637546
ApplicationsWestern Blot, ELISA, Other Application
Product group Antibodies
ReactivityVirus
TargetE7
Overview
- SupplierGeneTex
- Product NameHuman Papillomavirus type 16 E7 antibody [HL1821]
- Delivery Days Customer7
- Application Supplier Note*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA, Other Application
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1821
- Concentration2 mg/ml
- ConjugateUnconjugated
- Gene ID1489079
- Target nameE7
- Target descriptiontransforming protein E7
- Target synonymsHpV16gp2, transforming protein E7
- HostRabbit
- IsotypeIgG
- Protein IDP03129
- Protein NameProtein E7
- ReactivityVirus
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
Related products
Product group Antibodies
Anti-HPV16 E7 protein [69E2], Human IgG1, kappaAB03909-10.0
ApplicationsImmunoFluorescence, Western Blot, CLIA ChemiLuminecent ImmunoAssay, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityVirus
TargetE7
- SizePrice
![ELISA analysis of antigen using GTX60410 Human Papillomavirus type 16 E7 antibody [6F3].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng](https://www.genetex.com/upload/website/prouct_img/normal/GTX60410/GTX60410_20170912_ELISA_w_23061123_253.webp)
Product group Antibodies
References
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityVirus
TargetE7
- SizePrice
Product group Antibodies
References
HPV16 E7 Polyclonal AntibodyBS-10446R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetE7
- SizePrice
Product group Antibodies
HPV16 E7 Antibody (clone B616M)LS-C93017
ApplicationsWestern Blot, ELISA
ReactivityVirus
TargetE7
- SizePrice
Product group Antibodies
ApplicationsWestern Blot, ELISA
ReactivityVirus
TargetE7
- SizePrice
