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Immunohistochemistry analysis in human placenta and prostate tissues using Anti-HSD17B1 antibody. Corresponding HSD17B1 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human placenta and prostate tissues using Anti-HSD17B1 antibody. Corresponding HSD17B1 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human placenta and prostate tissues using Anti-HSD17B1 antibody. Corresponding HSD17B1 RNA-seq data are presented for the same tissues.

Anti-HSD17B1 Antibody

HPA065296
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetHSD17B1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HSD17B1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3292
  • Target name
    HSD17B1
  • Target description
    hydroxysteroid 17-beta dehydrogenase 1
  • Target synonyms
    17-beta-HSD, 20-alpha-HSD, E2DH, EDH17B2, EDHB17, HSD17, SDR28C1, 17-beta-hydroxysteroid dehydrogenase type 1, 17-beta-HSD 1, 20 alpha-hydroxysteroid dehydrogenase, estradiol 17-beta-dehydrogenase 1, placental 17-beta-hydroxysteroid dehydrogenase, short chain dehydrogenase/reductase family 28C member 1, short chain dehydrogenase/reductase family 28CE, member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14061
  • Protein Name
    Estradiol 17-beta-dehydrogenase 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of HSD17B1 using anti-HSD17B1 antibody (A02198). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Human Placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD17B1 antigen affinity purified polyclonal antibody (Catalog # A02198) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD17B1 at approximately 40KD. The expected band size for HSD17B1 is at 35KD.
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