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Immunohistochemical staining of human cervix, uterine shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human cervix, uterine shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human cervix, uterine shows moderate cytoplasmic positivity in glandular cells.

Anti-HSPA12A Antibody

HPA011273
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetHSPA12A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HSPA12A Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID259217
  • Target name
    HSPA12A
  • Target description
    heat shock protein family A (Hsp70) member 12A
  • Target synonyms
    heat shock 70 kDa protein 12A, heat shock 70kD protein 12A, heat shock 70kDa protein 12A, heat shock protein family A member 12A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43301
  • Protein Name
    Heat shock 70 kDa protein 12A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate All lanes: HSPA12A antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 75 kDa Observed band size: 75 kDa
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Figure 1. Western blot analysis of HSPA12A using anti-HSPA12A antibody (A13632-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA12A antigen affinity purified polyclonal antibody (Catalog # A13632-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPA12A at approximately 75 kDa. The expected band size for HSPA12A is at 75 kDa.
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Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & the Golgi apparatus.
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Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & the Golgi apparatus.
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