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Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in glandular cells.

Anti-HTATSF1 Antibody

HPA000504
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetHTATSF1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HTATSF1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27336
  • Target name
    HTATSF1
  • Target description
    HIV-1 Tat specific factor 1
  • Target synonyms
    cofactor required for Tat activation of HIV-1 transcription; dJ196E23.2; HIV TAT specific factor 1; HIV Tat-specific factor 1; TATSF1; TAT-SF1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43719
  • Protein Name
    HIV Tat-specific factor 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HTATSF1 using anti-HTATSF1 antibody (A07890-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HTATSF1 antigen affinity purified polyclonal antibody (Catalog # A07890-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HTATSF1 at approximately 140 kDa. The expected band size for HTATSF1 is at 86 kDa.
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