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Goat anti-HTATSF1 Antibody

EB07999
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityCanine, Human, Rat
TargetHTATSF1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-HTATSF1 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID27336
  • Target name
    HTATSF1
  • Target description
    HIV-1 Tat specific factor 1
  • Target synonyms
    TAT-SF1, TATSF1, dJ196E23.2, 17S U2 SnRNP complex component HTATSF1, HIV TAT specific factor 1, HIV Tat-specific factor 1, cofactor required for Tat activation of HIV-1 transcription
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_055315.2. GeneIDs all Nonhuman: 317612 (rat);. Purification: Antigen affinity purified. Names and symbols: HTATSF1; HIV-1 Tat specific factor 1 ; RP1-196E23.2; TAT-SF1; dJ196E23.2; HIV TAT specific factor 1; cofactor required for Tat activation of HIV-1 transcription
  • Reactivity
    Canine, Human, Rat
  • Reactivity Supplier
    Human, Rat, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Sikorski K, Mehta A, Inngjerdingen M, et al. A high-throughput pipeline for validation of antibodies. Nat Methods. 2018,15(11):909-912. doi: 10.1038/s41592-018-0179-8
    Read this paper

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Figure 1. Western blot analysis of HTATSF1 using anti-HTATSF1 antibody (A07890-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HTATSF1 antigen affinity purified polyclonal antibody (Catalog # A07890-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HTATSF1 at approximately 140 kDa. The expected band size for HTATSF1 is at 86 kDa.
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