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ProductsBack/Anti-Hu TROP2 Biotin

Anti-Hu TROP2 Biotin

1B-898
EXBIO
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Porcine
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Overview

  • Supplier
    EXBIO
  • Product Name
    Anti-Hu TROP2 Biotin
  • Delivery Days Customer
    7
  • Application Supplier Note
    Flow cytometry: Recommended dilution: 1-4 microg/ml.
  • Applications
    Flow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clone ID
    TrMab-6
  • Concentration
    1 mg/ml
  • Conjugate
    Biotin
  • Isotype
    IgG2b
  • Scientific Description
    TROP2 is a cell surface receptor that transduces calcium signals. It belongs to carcinoma-associated antigens. Mutations of TROP2 have been associated with gelatinous drop-like corneal dystrophy.
  • Reactivity
    Human, Mouse, Porcine
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TROP2/TACSTD2 using anti-TROP2/TACSTD2 antibody (A03569-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: rat pancreas tissue lysates, Lane 3: mouse pancreas tissue lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TROP2/TACSTD2 antigen affinity purified polyclonal antibody (Catalog # A03569-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TROP2/TACSTD2 at approximately 45-65 kDa. The expected band size for TROP2/TACSTD2 is at 36 kDa.
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