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ProductsBack/Biotin Anti-Human CD11a Antibody[TS1/22.1.1.13]

Biotin Anti-Human CD11a Antibody[TS1/22.1.1.13]

E-AB-F1055B
Elabscience
ApplicationsFlow Cytometry
Product group Antibodies
TargetITGAL
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Overview

  • Supplier
    Elabscience
  • Product Name
    Biotin Anti-Human CD11a Antibody[TS1/22.1.1.13]
  • Delivery Days Customer
    12
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    TS1/22.1.1.13
  • Concentration
    0.5 mg/ml
  • Conjugate
    Biotin
  • Gene ID3683
  • Target name
    ITGAL
  • Target description
    integrin subunit alpha L
  • Target synonyms
    CD11A, LFA-1, LFA1A, integrin alpha-L, CD11 antigen-like family member A, LFA-1 alpha, LFA-1A, antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide, integrin gene promoter, integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide), leukocyte adhesion glycoprotein LFA-1 alpha chain, leukocyte function-associated molecule 1 alpha chain, lymphocyte function-associated antigen 1, alpha polypeptide
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP20701
  • Protein Name
    Integrin alpha-L
  • Scientific Description
    CD11a is a 170-180 kD type I transmembrane glycoprotein also known as LFA-1alpha chain and integrin alphaL subunit. CD11a non-covalently associates with integrin beta2 (CD18) to form LFA-1. It is expressed on all leukocytes, including B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. It is absent on non-hematopoietic tissues and platelets. CD11a plays a central role in leukocyte cell-cell interactions and is important in lymphocyte costimulation. CD11a/CD18 binds to ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

MSDS

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Figure 1. Western blot analysis of CD11a using anti-CD11a antibody (A04466-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: JURKAT cell lysates, lane 2: CEM whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD11a antigen affinity purified polyclonal antibody (Catalog # A04466-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD11a at approximately 150KD. The expected band size for CD11a is at 129KD.
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