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Anti-Human CD18 Antibody, PE

136-18066
RayBiotech
ApplicationsFlow Cytometry
Product group Antibodies
TargetITGB2
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-Human CD18 Antibody, PE
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    IB4
  • Concentration
    5 ul/test
  • Conjugate
    RPE
  • Gene ID3689
  • Target name
    ITGB2
  • Target description
    integrin subunit beta 2
  • Target synonyms
    CD18, LAD, LCAMB, LFA-1, MAC-1, MF17, MFI7, integrin beta-2, cell surface adhesion glycoprotein (LFA-1/CR3/P150,959 beta subunit precursor), complement component 3 receptor 3 and 4 subunit, complement receptor C3 beta-subunit, integrin beta chain, beta 2, integrin, beta 2 (complement component 3 receptor 3 and 4 subunit), leukocyte cell adhesion molecule CD18, leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP05107
  • Protein Name
    Integrin beta-2
  • Scientific Description
    Human CD18 Antibody [clone: IB4], monoclonal mouse IgG2a. Validated for flow cytometry.
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD18/ITGB2 using anti-CD18/ITGB2 antibody (PB9141). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD18/ITGB2 antigen affinity purified polyclonal antibody (Catalog # PB9141) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD18/ITGB2 at approximately 90-100 kDa. The expected band size for CD18/ITGB2 is at 85 kDa.
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