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Western Blot analysis of HeLa cells using Integrin beta2 Polyclonal Antibody.
Western Blot analysis of HeLa cells using Integrin beta2 Polyclonal Antibody.
Western Blot analysis of HeLa cells using Integrin beta2 Polyclonal Antibody.

ITGB2 Antibody

CSB-PA005838
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetITGB2
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Overview

  • Supplier
    Cusabio
  • Product Name
    ITGB2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3689
  • Target name
    ITGB2
  • Target description
    integrin subunit beta 2
  • Target synonyms
    CD18, LAD, LCAMB, LFA-1, MAC-1, MF17, MFI7, integrin beta-2, cell surface adhesion glycoprotein (LFA-1/CR3/P150,959 beta subunit precursor), complement component 3 receptor 3 and 4 subunit, complement receptor C3 beta-subunit, integrin beta chain, beta 2, integrin, beta 2 (complement component 3 receptor 3 and 4 subunit), leukocyte cell adhesion molecule CD18, leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05107
  • Protein Name
    Integrin beta-2
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD18/ITGB2 using anti-CD18/ITGB2 antibody (PB9141). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD18/ITGB2 antigen affinity purified polyclonal antibody (Catalog # PB9141) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD18/ITGB2 at approximately 90-100 kDa. The expected band size for CD18/ITGB2 is at 85 kDa.
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