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ProductsBack/EBI3 antibody [MT140] (Biotin)
Sandwich ELISA analysis of human EBI3 protein using GTX02920 EBI3 antibody [MT23A14] as coating antibody and GTX02919-02 EBI3 antibody [MT140] (Biotin) as detecting antibody. Substrate : pNPP
Sandwich ELISA analysis of human EBI3 protein using GTX02920 EBI3 antibody [MT23A14] as coating antibody and GTX02919-02 EBI3 antibody [MT140] (Biotin) as detecting antibody. Substrate : pNPP
Sandwich ELISA analysis of human EBI3 protein using GTX02920 EBI3 antibody [MT23A14] as coating antibody and GTX02919-02 EBI3 antibody [MT140] (Biotin) as detecting antibody. Substrate : pNPP

EBI3 antibody [MT140] (Biotin)

GTX02919-02
GeneTex
ApplicationsELISA
DownloadDatasheet
Product group Antibodies
TargetEBI3
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Overview

  • Supplier
    GeneTex
  • Product Name
    EBI3 antibody [MT140] (Biotin)
  • Delivery Days Customer
    9
  • Application Supplier Note
    *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MT140
  • Concentration
    0.5 mg/ml
  • Conjugate
    Biotin
  • Gene ID10148
  • Target name
    EBI3
  • Target description
    Epstein-Barr virus induced 3
  • Target synonyms
    cytokine receptor; EBV-induced gene 3 protein; Epstein-Barr virus induced gene 3; epstein-Barr virus-induced gene 3 protein; IL27 subunit; IL-27 subunit beta; IL27B; IL-27B; IL35 subunit; IL35B; interleukin-27 subunit beta
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ14213
  • Protein Name
    Interleukin-27 subunit beta
  • Scientific Description
    This gene was identified by its induced expression in B lymphocytes in response Epstein-Barr virus infection. It encodes a secreted glycoprotein belonging to the hematopoietin receptor family, and heterodimerizes with a 28 kDa protein to form interleukin 27 (IL-27). IL-27 regulates T cell and inflammatory responses, in part by activating the Jak/STAT pathway of CD4+ T cells. [provided by RefSeq, Sep 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EBI3 using anti-EBI3 antibody (M07190-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: rat spleen tissue lysates, Lane 5: rat thymus tissue lysates, Lane 7: mouse spleen tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EBI3 antigen affinity purified monoclonal antibody (Catalog # M07190-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EBI3 at approximately 30 kDa. The expected band size for EBI3 is at 25,30 kDa.
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