Figure 1. Western blot analysis of IFT88 using anti-IFT88 antibody (A06814-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFT88 antigen affinity purified polyclonal antibody (Catalog # A06814-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFT88 at approximately 94KD. The expected band size for IFT88 is at 94KD.
Figure 1. Western blot analysis of IFT88 using anti-IFT88 antibody (A06814-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFT88 antigen affinity purified polyclonal antibody (Catalog # A06814-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFT88 at approximately 94KD. The expected band size for IFT88 is at 94KD.
Anti-IFT88 Antibody Picoband(r)
A06814-1-DYLIGHT594
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetIFT88
Overview
- SupplierBoster Bio
- Product NameAnti-IFT88 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateOther Conjugate
- Gene ID8100
- Target nameIFT88
- Target descriptionintraflagellar transport 88
- Target synonymsD13S1056E; DAF19; hTg737; intraflagellar transport 88 homolog; intraflagellar transport protein 88 homolog; polaris homolog; probe hTg737 (polycystic kidney disease, autosomal recessive); recessive polycystic kidney disease protein Tg737 homolog; testicular tissue protein Li 93; tetratricopeptide repeat domain 10; tetratricopeptide repeat protein 10; TG737; TPR repeat protein 10; TTC10
- HostRabbit
- IsotypeIgG
- Protein IDQ13099
- Protein NameIntraflagellar transport protein 88 homolog
- Scientific DescriptionBoster Bio Anti-IFT88 Antibody Picoband® catalog # A06814-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203