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ProductsBack/Anti-IL-12/23 [ABT-874 (Briakinumab)]

Anti-IL-12/23 [ABT-874 (Briakinumab)]

Ab00722-10.0
Absolute Antibody
ApplicationsNeutralisation/Blocking
Product group Antibodies
TargetIL12B
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-IL-12/23 [ABT-874 (Briakinumab)]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody binds to and neutralises human IL12 and IL23.
  • Applications
    Neutralisation/Blocking
  • Applications Supplier
    block
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ABT-874 (Briakinumab)
  • Gene ID3593
  • Target name
    IL12B
  • Target description
    interleukin 12B
  • Target synonyms
    CLMF; CLMF p40; CLMF2; cytotoxic lymphocyte maturation factor 40 kDa subunit; IL-12 subunit p40; IL12, subunit p40; IL-12B; IMD28; IMD29; interleukin 12, p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin-12 beta chain; interleukin-12 subunit beta; natural killer cell stimulatory factor, 40 kD subunit; NK cell stimulatory factor chain 2; NKSF; NKSF2
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP29460
  • Protein Name
    Interleukin-12 subunit beta
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    This antibody was generated by phage display technology.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL12B using anti-IL12B antibody (A01152-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse NIH3T3 cell lysates, Lane 2: human A549 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL12B antigen affinity purified polyclonal antibody (Catalog # A01152-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL12B at approximately 37KD. The expected band size for IL12B is at 37KD.
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