Anti-IL-12p40 [Antibody 80]

Absolute Antibody

Anti-IL-12p40 [Antibody 80]

9

This antibody neutralized the binding of IL-12 to IL-12Rbeta2 and IL-23 to IL-23R but did not inhibit the binding of IL-12 or IL-23 or IL-12p40 or IL-12p80 to IL-12Rbeta1. The human IgG version of this antibody binds IL-12 and IL-23 with a binding affinity of Kd= 292 pM and Kd= 280 pM respectively. This antibody was reported to inhibit IL-12 induced IFN-gamma release by NK-92 cells and human PBMCs. This antibody can also inhibit IL-23 induced IL-17 secretion by murine splenocytes. The therapeutic efficacy of this antibody was studied in the skin inflammation mouse model. This antibody was reported to significantly decrease epidermal thickness and reduced erythema and induration caused by IL-23. This antibody is capable of detecting recombinant IL-23 and native IL-23 produced by SAC stimulated PBMCs in an ELISA. This antibody showed reduced binding to IL-23 mutants D265A and R266A in an ELISA (US8563697).

Functional Assay, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

Antibody 80

IL12B

interleukin 12B

CLMF, CLMF2, IL-12B, IMD28, IMD29, NKSF, NKSF2, interleukin-12 subunit beta, CLMF p40, IL-12 subunit p40, IL12, subunit p40, NK cell stimulatory factor chain 2, cytotoxic lymphocyte maturation factor 40 kDa subunit, interleukin 12, p40, interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40), interleukin-12 beta chain, natural killer cell stimulatory factor, 40 kD subunit

Human

IgG1

Interleukin-12 subunit beta

This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203