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Anti-IL-17A [MM17F3]

Ab01266-1.1
Absolute Antibody
ApplicationsELISA, Neutralisation/Blocking
Product group Antibodies
TargetIl17a
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-IL-17A [MM17F3]
  • Delivery Days Customer
    7
  • Application Supplier Note
    The specificity of this antibody has been confirmed in ELISA analysis (Uyttenhove et al, 2006). This antibody inhibits IL-17A-induced IL-6 production by a Cl1D fibroblastic cell line in vitro (Uyttenhove et al, 2006). This antibody has been shown to prevent experimental autoimmune encephalomyelitis (EAE) disease development in vivo (Uyttenhove et al, 2006), as well as protecting C57Bl/6 mice from myelin oligdendrocyte glycoprotein (MOG)-induced EAE (Uyttenhove et al, 2007). Additionally, administration of this antibody inhibits collagen-induced arthritis (CIA) in IFN-gamma receptor knockout mice (Kelchtermans et al, 2009).
  • Applications
    ELISA, Neutralisation/Blocking
  • Applications Supplier
    ELISA; blocking; therapeutic
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MM17F3
  • Gene ID16171
  • Target name
    Il17a
  • Target description
    interleukin 17A
  • Target synonyms
    Ctl; Ctla; Ctla8; Ctla-8; cytotoxic T-lymphocyte-associated antigen 8; Il; IL-; Il17; IL-17; IL-17A; interleukin-17A
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ62386
  • Protein Name
    Interleukin-17A
  • Reactivity Supplier
    Mouse
  • Reactivity Supplier Note
    This antibody was raised by immunising C57Bl/6 mice with IL-17A cross-linked to ovalbumin to generate a highly immunogenic complex.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL17 using anti-IL17 antibody (A00421-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17 antigen affinity purified polyclonal antibody (Catalog # A00421-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17 at approximately 27KD. The expected band size for IL17 is at 17KD.
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