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Anti-mouse IL-17A mAb (IL17-I), unconjugated

Anti-mouse IL-17A mAb (IL17-I), unconjugated

3521-3
Mabtech
ApplicationsELISA, ELISpot Assay
Product group Antibodies
TargetIl17a
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Overview

  • Supplier
    Mabtech
  • Product Name
    Anti-mouse IL-17A mAb (IL17-I), unconjugated
  • Delivery Days Customer
    9
  • Applications
    ELISA, ELISpot Assay
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    IL17-I
  • Conjugate
    Unconjugated
  • Gene ID16171
  • Target name
    Il17a
  • Target description
    interleukin 17A
  • Target synonyms
    Ctla-8, Ctla8, IL-17, IL-17A, Il17, interleukin-17A, cytotoxic T-lymphocyte-associated antigen 8
  • Host
    Rat
  • Isotype
    IgG1
  • Scientific Description
    Validated for our assays. - This monoclonal antibody enables specific detection of mouse IL-17A in immunoassays such as ELISpot, FluoroSpot, and ELISA.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL17 using anti-IL17 antibody (A00421-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17 antigen affinity purified polyclonal antibody (Catalog # A00421-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17 at approximately 27KD. The expected band size for IL17 is at 17KD.
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