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ProductsBack/FITC Anti-Mouse IL-17A Antibody [17F3]

FITC Anti-Mouse IL-17A Antibody [17F3]

AGEL3102
Assay Genie
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityMouse
TargetIl17a
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Overview

  • Supplier
    Assay Genie
  • Product Name
    FITC Anti-Mouse IL-17A Antibody [17F3]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry
  • Applications Supplier
    FCM
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    17F3
  • Conjugate
    FITC
  • Gene ID16171
  • Target name
    Il17a
  • Target description
    interleukin 17A
  • Target synonyms
    Ctla-8, Ctla8, IL-17, IL-17A, Il17, interleukin-17A, cytotoxic T-lymphocyte-associated antigen 8
  • Host
    Mouse
  • Protein IDQ62386
  • Protein Name
    Interleukin-17A
  • Scientific Description
    FITC Anti-Mouse IL-17A Antibody [17F3] is a high-performance monoclonal that specifically targets Mouse IL-17A, delivering exceptional specificity and reliability for your research applications. Rigorously validated for FCM, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Mouse samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100 Tests format to meet your experimental needs. For optimal performance, store at 4°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Mouse
  • Reactivity
    Mouse
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of IL17 using anti-IL17 antibody (A00421-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17 antigen affinity purified polyclonal antibody (Catalog # A00421-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17 at approximately 27KD. The expected band size for IL17 is at 17KD.
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