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Anti-IL-18 [125-2H]

Ab00140-1.1
Absolute Antibody
ApplicationsImmunoFluorescence, ImmunoPrecipitation, ELISA, Neutralisation/Blocking
Product group Antibodies
TargetIL18
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-IL-18 [125-2H]
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, ELISA, Neutralisation/Blocking
  • Applications Supplier
    ELISA; IF; IP; NTRL
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    125-2H
  • Gene ID3606
  • Target name
    IL18
  • Target description
    interleukin 18
  • Target synonyms
    IGIF, IL-18, IL-1g, IL1F4, interleukin-18, IFN-gamma-inducing factor, IL-1 gamma, iboctadekin, interleukin 18 (interferon-gamma-inducing factor), interleukin-1 gamma
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ14116
  • Protein Name
    Interleukin-18
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    Fusion of splenocytes from mice immunized with human IL-18, with SP2/O mouse myeloma cells.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL18 using anti-IL18 antibody (PB9006). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL18 antigen affinity purified polyclonal antibody (Catalog # PB9006) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL18 at approximately 22 kDa. The expected band size for IL18 is at 22 kDa.
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