IL18 antibody [HL1761] detects IL18 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human tonsil. IL18 stained by IL18 antibody [HL1761] (GTX637411) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL18 antibody [HL1761] (GTX637411) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637411/GTX637411_44837_20221028_WB_TPM_watermark_22110201_106.webp "Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL18 antibody [HL1761] (GTX637411) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) (3.47-0.05 nM). Coated protein was probed with IL18 antibody [HL1761] (GTX637411) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637411/GTX637411_44837_20230324_ELISA_Indirect_23032819_734.webp "Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) (3.47-0.05 nM). Coated protein was probed with IL18 antibody [HL1761] (GTX637411) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.")
![Human IL18 protein was separated by 12% SDS-PAGE, and the membrane was blotted with IL18 antibody [HL1761] (GTX637411) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637411/GTX637411_44837_20230505_WB_Ag_23050918_211.webp "Human IL18 protein was separated by 12% SDS-PAGE, and the membrane was blotted with IL18 antibody [HL1761] (GTX637411) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Sandwich ELISA detection of recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) using antibodies as below. Capture: IL18 antibody [HL1859] (GTX637584) and IL18 antibody [HL1761] (GTX637411) (5 μg/mL) Detection: HRP-conjugated IL18 antibody [HL1859] (GTX637584), IL18 antibody [HL1761] (GTX637411) and IL18 antibody [HL2523] (GTX638884) (1 μg/mL) Please notice that GTX637584 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637411. Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637411/GTX637411_44837_20230915_ELISA_PAIR_Multiple_23091901_295.webp "Sandwich ELISA detection of recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) using antibodies as below. Capture: IL18 antibody [HL1859] (GTX637584) and IL18 antibody [HL1761] (GTX637411) (5 μg/mL) Detection: HRP-conjugated IL18 antibody [HL1859] (GTX637584), IL18 antibody [HL1761] (GTX637411) and IL18 antibody [HL2523] (GTX638884) (1 μg/mL) Please notice that GTX637584 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637411. Please contact us for custom HRP-conjugated antibody.")
![Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL18 antibody [HL1761] (GTX637411) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637411/GTX637411_44837_20231013_WB_shRNA_watermark_23101921_602.webp "Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL18 antibody [HL1761] (GTX637411) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
IL18 antibody [HL1761] detects IL18 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human tonsil. IL18 stained by IL18 antibody [HL1761] (GTX637411) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
IL18 antibody [HL1761]
GTX637411
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetIL18
Overview
- SupplierGeneTex
- Product NameIL18 antibody [HL1761]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1761
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3606
- Target nameIL18
- Target descriptioninterleukin 18
- Target synonymsIGIF, IL-18, IL-1g, IL1F4, interleukin-18, IFN-gamma-inducing factor, IL-1 gamma, iboctadekin, interleukin 18 (interferon-gamma-inducing factor), interleukin-1 gamma
- HostRabbit
- IsotypeIgG
- Protein IDQ14116
- Protein NameInterleukin-18
- Scientific DescriptionThe protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
Related products
Product group Antibodies
Anti-IL-18 [125-2H]Ab00140-1.1
ApplicationsImmunoFluorescence, ImmunoPrecipitation, ELISA, Neutralisation/Blocking
ReactivityHuman
TargetIL18
- SizePrice
Product group Antibodies
Anti-IL18 Antibody130-10603
ApplicationsELISA
ReactivityHuman
TargetIL18
- SizePrice
Product group Antibodies
IL18 antibodyGTX03734
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityMouse, Rat
TargetIL18
- SizePrice
Product group Antibodies
IL18 Polyclonal AntibodyCAC13342
ApplicationsELISA, ImmunoHistoChemistry
TargetIL18
- SizePrice
Product group Antibodies
Anti-IL18 AntibodyA30073
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
- SizePrice
Product group Antibodies
References
IL18 antibodyGTX32675
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetIL18
- SizePrice
![Sandwich ELISA detection of recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) using antibodies as below. Capture: IL18 antibody [HL2938] (GTX640322) (5 μg/mL) Detection: HRP-conjugated IL18 antibody [HL1859] (GTX637584) (1 μg/mL) Please notice that GTX637584 needs to be conjugated to HRP to function as the detection antibody when paired with GTX640322. Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640322/GTX640322_45460_20240712_ELISA_PAIR_24071520_952.webp)
Product group Antibodies
IL18 antibody [HL2938]GTX640322
ApplicationsELISA
ReactivityHuman
TargetIL18
- SizePrice
Product group Antibodies
IL18 Polyclonal Antibodybs-4988R
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetIL18
- SizePrice
