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Goat anti-IL18

EB09393
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetIL18
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-IL18 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the golgi and cytoplasm of A431and U2OS cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID3606
  • Target name
    IL18
  • Target description
    interleukin 18
  • Target synonyms
    iboctadekin; IFN-gamma-inducing factor; IGIF; IL-1 gamma; IL-18; IL1F4; IL-1g; interleukin 18 (interferon-gamma-inducing factor); interleukin-1 gamma; interleukin-18
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001553.1; NP_001230140.1. GeneIDs all Nonhuman: 29197 (rat);. Purification: Antigen affinity purified. Names and symbols: IL18; interleukin 18 (interferon-gamma-inducing factor); IGIF; IL-18; IL-1g; IL1F4; MGC12320; IL-1 gamma; interferon-gamma-inducing factor; interleukin 18; interleukin-1 gamma; interleukin-18
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL18 using anti-IL18 antibody (PB9006). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL18 antigen affinity purified polyclonal antibody (Catalog # PB9006) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL18 at approximately 22 kDa. The expected band size for IL18 is at 22 kDa.
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