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Anti-IL-6 Antibody

130-10060
RayBiotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-IL-6 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1B1-B10-D2
  • Host
    Mouse
  • Scientific Description
    Mouse Anti-Human IL-6 Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL-6 using anti-IL-6 antibody (RP1012). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human IL6 protein 10 ng, Lane 2: recombinant human IL6 protein 5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-6 antigen affinity purified polyclonal antibody (Catalog # RP1012) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
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