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Formalin-fixed and paraffin embedded Human oral mucosa labeled with Anti-IL-6(human) Polyclonal Antibody, Unconjugated (bs-0781R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded Human oral mucosa labeled with Anti-IL-6(human) Polyclonal Antibody, Unconjugated (bs-0781R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded Human oral mucosa labeled with Anti-IL-6(human) Polyclonal Antibody, Unconjugated (bs-0781R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

IL-6 Polyclonal Antibody

BS-0781R
Bioss Antibodies
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetIL6
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    IL-6 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3569
  • Target name
    IL6
  • Target description
    interleukin 6
  • Target synonyms
    B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor; HGF; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; IL-6; interferon beta-2; interleukin BSF-2; interleukin-6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05231
  • Protein Name
    Interleukin-6
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Significance of interstitial tumor-associated macrophages in the progression of lung adenocarcinoma. Sun BS et al., 2016 Dec, Oncol Lett
    Read more
  • Electroacupuncture modulated the inflammatory reaction in MCAO rats via inhibiting the TLR4/NF-kappaB signaling pathway in microglia. Han B et al., 2015, Int J Clin Exp Pathol
    Read more
  • Interstitial tumor-associated macrophages combined with tumor-derived colony-stimulating factor-1 and interleukin-6, a novel prognostic biomarker in non-small cell lung cancer. Pei BX et al., 2014 Oct, J Thorac Cardiovasc Surg
    Read more

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Figure 1. Western blot analysis of IL-6 using anti-IL-6 antibody (RP1012). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human IL6 protein 10 ng, Lane 2: recombinant human IL6 protein 5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-6 antigen affinity purified polyclonal antibody (Catalog # RP1012) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
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