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Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in squamous epithelial cells.

Anti-IL1RN Antibody

HPA001482
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetIL1RN
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-IL1RN Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3557
  • Target name
    IL1RN
  • Target description
    interleukin 1 receptor antagonist
  • Target synonyms
    CRMO2, DIRA, ICIL-1RA, IL-1RN, IL-1ra, IL-1ra3, IL1F3, IL1RA, IRAP, MVCD4, interleukin-1 receptor antagonist protein, IL1 inhibitor, intracellular IL-1 receptor antagonist type II, intracellular interleukin-1 receptor antagonist (icIL-1ra), type II interleukin-1 receptor antagonist
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18510
  • Protein Name
    Interleukin-1 receptor antagonist protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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FAQ

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Figure 1. Western blot analysis of IL1RA using anti-IL1RA antibody (A00651-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1RA antigen affinity purified polyclonal antibody (Catalog # A00651-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL1RA at approximately 20 kDa. The expected band size for IL1RA is at 20 kDa.
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