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Western blot All lanes: IL1RN antibody at 2microg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 21, 18, 20, 17 kDa Observed band size: 17 kDa
Western blot All lanes: IL1RN antibody at 2microg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 21, 18, 20, 17 kDa Observed band size: 17 kDa
Western blot All lanes: IL1RN antibody at 2microg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 21, 18, 20, 17 kDa Observed band size: 17 kDa

IL1RN Antibody

CSB-PA06677A0RB
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetIL1RN
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Overview

  • Supplier
    Cusabio
  • Product Name
    IL1RN Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3557
  • Target name
    IL1RN
  • Target description
    interleukin 1 receptor antagonist
  • Target synonyms
    DIRA; ICIL-1RA; IL1 inhibitor; IL1F3; IL1RA; IL-1ra; IL-1ra3; IL-1RN; interleukin-1 receptor antagonist protein; intracellular IL-1 receptor antagonist type II; intracellular interleukin-1 receptor antagonist (icIL-1ra); IRAP; MVCD4; type II interleukin-1 receptor antagonist
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18510
  • Protein Name
    Interleukin-1 receptor antagonist protein
  • Scientific Description
    Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL1RA using anti-IL1RA antibody (A00651-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1RA antigen affinity purified polyclonal antibody (Catalog # A00651-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL1RA at approximately 20 kDa. The expected band size for IL1RA is at 20 kDa.
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