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Anti-IL23A (Center) Antibody

102-25235
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetIL23A
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-IL23A (Center) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB56752
  • Gene ID51561
  • Target name
    IL23A
  • Target description
    interleukin 23 subunit alpha
  • Target synonyms
    IL-23; IL-23 subunit alpha; IL-23A; IL-23-A; IL23P19; IL-23p19; interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; interleukin-23 subunit p19; interleukin-six, G-CSF related factor; JKA3 induced upon T-cell activation; P19; SGRF
  • Host
    Rabbit
  • Protein IDQ9NPF7
  • Protein Name
    Interleukin-23 subunit alpha
  • Scientific Description
    Rabbit Anti-IL23A (Center) Antibody, 200 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL23 using anti-IL23 antibody (A01097-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PANC-1 cell lysates, Lane 3: rat lymph tissue lysates, Lane 4: rat small intestine tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: rat ovary tissue lysates, Lane 7: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL23 antigen affinity purified polyclonal antibody (Catalog # A01097-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL23 at approximately 19KD. The expected band size for IL23 is at 21KD.
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