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Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA06719A0Rb at dilution of 1:100
Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA06719A0Rb at dilution of 1:100
Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA06719A0Rb at dilution of 1:100

IL23A Antibody

CSB-PA06719A0RB
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetIL23A
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Overview

  • Supplier
    Cusabio
  • Product Name
    IL23A Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51561
  • Target name
    IL23A
  • Target description
    interleukin 23 subunit alpha
  • Target synonyms
    IL-23, IL-23A, IL23P19, P19, SGRF, interleukin-23 subunit alpha, IL-23 subunit alpha, IL-23-A, IL-23p19, JKA3 induced upon T-cell activation, interleukin 23 p19 subunit, interleukin 23, alpha subunit p19, interleukin-23 subunit p19, interleukin-six, G-CSF related factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NPF7
  • Protein Name
    Interleukin-23 subunit alpha
  • Scientific Description
    Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IL23 using anti-IL23 antibody (A01097-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PANC-1 cell lysates, Lane 3: rat lymph tissue lysates, Lane 4: rat small intestine tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: rat ovary tissue lysates, Lane 7: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL23 antigen affinity purified polyclonal antibody (Catalog # A01097-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL23 at approximately 19KD. The expected band size for IL23 is at 21KD.
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