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Sandwich ELISA analysis of human IL-23 protein using GTX02957 IL12B / IL12 p40 antibody [MT86 + MT221] as coating antibody and GTX02983-02 L23A / IL23 p19 antibody [MT155] (Biotin) as detecting antibody. Substrate : pNPP
Sandwich ELISA analysis of human IL-23 protein using GTX02957 IL12B / IL12 p40 antibody [MT86 + MT221] as coating antibody and GTX02983-02 L23A / IL23 p19 antibody [MT155] (Biotin) as detecting antibody. Substrate : pNPP
Sandwich ELISA analysis of human IL-23 protein using GTX02957 IL12B / IL12 p40 antibody [MT86 + MT221] as coating antibody and GTX02983-02 L23A / IL23 p19 antibody [MT155] (Biotin) as detecting antibody. Substrate : pNPP

L23A / IL23 p19 antibody [MT155] (Biotin)

GTX02983-02
GeneTex
ApplicationsELISA
Product group Antibodies
TargetIL23A
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Overview

  • Supplier
    GeneTex
  • Product Name
    L23A / IL23 p19 antibody [MT155] (Biotin)
  • Delivery Days Customer
    9
  • Application Supplier Note
    *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MT155
  • Concentration
    0.5 mg/ml
  • Conjugate
    Biotin
  • Gene ID51561
  • Target name
    IL23A
  • Target description
    interleukin 23 subunit alpha
  • Target synonyms
    IL-23; IL-23 subunit alpha; IL-23A; IL-23-A; IL23P19; IL-23p19; interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; interleukin-23 subunit p19; interleukin-six, G-CSF related factor; JKA3 induced upon T-cell activation; P19; SGRF
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ9NPF7
  • Protein Name
    Interleukin-23 subunit alpha
  • Scientific Description
    This gene encodes a subunit of the heterodimeric cytokine interleukin 23 (IL23). IL23 is composed of this protein and the p40 subunit of interleukin 12 (IL12B). The receptor of IL23 is formed by the beta 1 subunit of IL12 (IL12RB1) and an IL23 specific subunit, IL23R. Both IL23 and IL12 can activate the transcription activator STAT4, and stimulate the production of interferon-gamma (IFNG). In contrast to IL12, which acts mainly on naive CD4(+) T cells, IL23 preferentially acts on memory CD4(+) T cells. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL23 using anti-IL23 antibody (A01097-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PANC-1 cell lysates, Lane 3: rat lymph tissue lysates, Lane 4: rat small intestine tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: rat ovary tissue lysates, Lane 7: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL23 antigen affinity purified polyclonal antibody (Catalog # A01097-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL23 at approximately 19KD. The expected band size for IL23 is at 21KD.
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