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Anti-IL2R beta [5H4]

Ab01372-23.0
Absolute Antibody
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityMouse
TargetIl2rb
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-IL2R beta [5H4]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody has been used in flow cytometric analysis of IL-2R beta expression by thymocytes, lymph node cells and 3B4.15 T hybridoma cells (Ligons et al, 2012), as well as NK cells (He et al, 1996; Frenkel et al, 2016; Put et al, 2016; Elkabets et al, 2009).
  • Applications
    Flow Cytometry
  • Applications Supplier
    FC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5H4
  • Gene ID16185
  • Target name
    Il2rb
  • Target description
    interleukin 2 receptor, beta chain
  • Target synonyms
    CD122, IL-15Rbeta, IL15Rbeta, Il-2/15Rbeta, Il-2Rbeta, p70, interleukin-2 receptor subunit beta, IL-15 receptor beta chain, IL-2 receptor beta chain, IL-2 receptor subunit beta, IL-2/15 receptor-beta, IL-2R subunit beta, IL-2RB, high affinity IL-2 receptor subunit beta, p70-75
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP16297
  • Protein Name
    Interleukin-2 receptor subunit beta
  • Scientific Description
    This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity
    Mouse
  • Reactivity Supplier
    Mouse
  • Reactivity Supplier Note
    This antibody was raised by immunising Lewis rats with transfected rat myeloma cell line YB2/0, expressing truncated murine IL-2R beta.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL2RB using anti-IL2RB antibody (PB9761). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: Mouse Lung Tissue Lysate, Lane 2: Mouse Spleen Tissue Lysate, Lane 3: Mouse Testis Tissue Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL2RB antigen affinity purified polyclonal antibody (Catalog # PB9761) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL2RB at approximately 75 kDa. The expected band size for IL2RB is at 75 kDa.
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